LOCUS       pSTV2_N-BirA_pDe        9311 bp    DNA     circular     30-AUG-2018
DEFINITION  .
ACCESSION   urn.local...7w-9fz0ltg
VERSION
KEYWORDS    .
SOURCE      null
  ORGANISM  
            .
COMMENT     Created by Clontech Laboratories Inc. http://www.clontech.com Cat.
            No. 632158,632162,632157,632163.
FEATURES             Location/Qualifiers
     LTR             1..635
                     /label="5'\LTR"
     misc_binding    636..653
                     /label="PBS"
     misc_feature    685..822
                     /label="Packaging\signal"
     misc_binding    1303..1536
                     /label="RRE"
     misc_feature    2028..2151
                     /label="cPPT/CTS"
     promoter        2205..2520
                     /modified_by="User"
                     /label="TREtight"
     CDS             2601..3563
                     /ApEinfo_fwdcolor="#2474dd"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="BirA"
     CDS             3594..3617
                     /ApEinfo_fwdcolor="#006ddd"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="FLAG"
     attR1           3630..3754
                     /ApEinfo_fwdcolor="cyan"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="ATTR1"
     CDS             3863..4522
                     /ApEinfo_fwdcolor="#00dd14"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="Chlorm"
     CDS             4864..5169
                     /ApEinfo_fwdcolor="#00dd14"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /label="ccdB"
     attR2           5210..5334
                     /created_by="User"
                     /modified_by="User"
                     /label="ATTR2"
     WPRE            5355..5930
                     /ApEinfo_fwdcolor="#008cff"
                     /ApEinfo_revcolor="green"
                     /ApEinfo_graphicformat="arrow_data {{0 1 2 0 0 -1} {} 0}
                     width 5 offset 0"
                     /modified_by="User"
                     /label="WPRE"
     LTR             6027..6663
                     /label="3'\LTR"
     rep_origin      complement(7132..7805)
                     /label="pUC\origin"
     CDS             complement(7950..8946)
                     /label="Amp(R)"
ORIGIN      
        1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
       61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
      121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
      181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
      241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
      301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
      361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
      421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
      481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
      541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
      601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
      661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
      721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
      781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
      841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
      901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
      961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
     1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
     1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
     1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
     1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
     1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
     1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
     1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
     1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
     1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
     1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
     1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
     1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
     1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
     1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
     1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
     1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
     1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccttt aaaagaaaag
     2041 gggggattgg ggggtacagt gcaggggaaa gaatagtaga cataatagca acagacatac
     2101 aaactaaaga attacaaaaa caaattacaa aaattcaaaa ttttcgggtt tattacaggg
     2161 acagcagaga tccagtttat cgatgaggcc ctttcgtctt cactcgagtt tactccctat
     2221 cagtgataga gaacgtatgt cgagtttact ccctatcagt gatagagaac gatgtcgagt
     2281 ttactcccta tcagtgatag agaacgtatg tcgagtttac tccctatcag tgatagagaa
     2341 cgtatgtcga gtttactccc tatcagtgat agagaacgta tgtcgagttt atccctatca
     2401 gtgatagaga acgtatgtcg agtttactcc ctatcagtga tagagaacgt atgtcgaggt
     2461 aggcgtgtac ggtgggaggc ctatataagc agagctcgtt tagtgaaccg tcagatcgcc
     2521 tggagaagga tccgcggccg cgccggctct agatcgcgaa cgcgagcttg gtaccgagct
     2581 cggatccagc tagcgccacc atgaaggaca acaccgtgcc cctgaagctg atcgccctgc
     2641 tggccaacgg cgagttccac agcggcgagc agctgggcga gaccctgggc atgagccggg
     2701 ccgccatcaa caagcacatc cagaccctgc gggactgggg cgtggacgtg ttcaccgtgc
     2761 ccggcaaggg ctacagcctg cccgagccca tccagctgct gaacgccaag cagatcctgg
     2821 gccagctgga cggcggcagc gtggccgtgc tgcccgtgat cgacagcacc aaccagtacc
     2881 tgctggaccg gatcggcgag ctgaagagcg gcgacgcctg catcgccgag taccagcagg
     2941 ccggccgggg cggccggggc cggaagtggt tcagcccctt cggcgccaac ctgtacctga
     3001 gcatgttctg gcggctggag cagggccccg ccgccgccat cggcctgagc ctggtgatcg
     3061 gcatcgtgat ggccgaggtg ctgcggaagc tgggcgccga caaggtgcgg gtgaagtggc
     3121 ccaacgacct gtacctgcag gaccggaagc tggccggcat cctggtggag ctgaccggca
     3181 agaccggcga cgccgcccag atcgtgatcg gcgccggcat caacatggcc atgcggcggg
     3241 tggaggagag cgtggtgaac cagggctgga tcaccctgca ggaggccggc atcaacctgg
     3301 accggaacac cctggccgcc atgctgatcc gggagctgcg ggccgccctg gagctgttcg
     3361 agcaggaggg cctggccccc tacctgagcc ggtgggagaa gctggacaac ttcatcaacc
     3421 ggcccgtgaa gctgatcatc ggcgacaagg agatcttcgg catcagccgg ggcatcgaca
     3481 agcagggcgc cctgctgctg gagcaggacg gcatcatcaa gccctggatg ggcggcgaga
     3541 tcagcctgcg gagcgccgag aaggggggat ctggccccgg cggcggcgcg ccagactaca
     3601 aagacgatga cgataaacta ggcgcgccca caagtttgta caaaaaagct gaacgagaaa
     3661 cgtaaaatga tataaatatc aatatattaa attagatttt gcataaaaaa cagactacat
     3721 aatactgtaa aacacaacat atccagtcac tatggcggcc gcattaggca ccccaggctt
     3781 tacactttat gcttccggct cgtataatgt gtggattttg agttaggatc cgtcgagatt
     3841 ttcaggagct aaggaagcta aaatggagaa aaaaatcact ggatatacca ccgttgatat
     3901 atcccaatgg catcgtaaag aacattttga ggcatttcag tcagttgctc aatgtaccta
     3961 taaccagacc gttcagctgg atattacggc ctttttaaag accgtaaaga aaaataagca
     4021 caagttttat ccggccttta ttcacattct tgcccgcctg atgaatgctc atccggaatt
     4081 ccgtatggca atgaaagacg gtgagctggt gatatgggat agtgttcacc cttgttacac
     4141 cgttttccat gagcaaactg aaacgttttc atcgctctgg agtgaatacc acgacgattt
     4201 ccggcagttt ctacacatat attcgcaaga tgtggcgtgt tacggtgaaa acctggccta
     4261 tttccctaaa gggtttattg agaatatgtt tttcgtctca gccaatccct gggtgagttt
     4321 caccagtttt gatttaaacg tggccaatat ggacaacttc ttcgcccccg ttttcaccat
     4381 gggcaaatat tatacgcaag gcgacaaggt gctgatgccg ctggcgattc aggttcatca
     4441 tgccgtttgt gatggcttcc atgtcggcag aatgcttaat gaattacaac agtactgcga
     4501 tgagtggcag ggcggggcgt aaagatctgg atccggctta ctaaaagcca gataacagta
     4561 tgcgtatttg cgcgctgatt tttgcggtat aagaatatat actgatatgt atacccgaag
     4621 tatgtcaaaa agaggtatgc tatgaagcag cgtattacag tgacagttga cagcgacagc
     4681 tatcagttgc tcaaggcata tatgatgtca atatctccgg tctggtaagc acaaccatgc
     4741 agaatgaagc ccgtcgtctg cgtgccgaac gctggaaagc ggaaaatcag gaagggatgg
     4801 ctgaggtcgc ccggtttatt gaaatgaacg gctcttttgc tgacgagaac aggggctggt
     4861 gaaatgcagt ttaaggttta cacctataaa agagagagcc gttatcgtct gtttgtggat
     4921 gtacagagtg atattattga cacgcccggg cgacggatgg tgatccccct ggccagtgca
     4981 cgtctgctgt cagataaagt ctcccgtgaa ctttacccgg tggtgcatat cggggatgaa
     5041 agctggcgca tgatgaccac cgatatggcc agtgtgccgg tctccgttat cggggaagaa
     5101 gtggctgatc tcagccaccg cgaaaatgac atcaaaaacg ccattaacct gatgttctgg
     5161 ggaatataaa tgtcaggctc ccttatacac agccagtctg caggtcgacc atagtgactg
     5221 gatatgttgt gttttacagt attatgtagt ctgtttttta tgcaaaatct aatttaatat
     5281 attgatattt atatcatttt acgtttctcg ttcagctttc ttgtacaaag tggtgtgact
     5341 cgagcagtcg acaatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
     5401 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
     5461 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
     5521 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
     5581 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
     5641 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
     5701 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaagct gacgtccttt
     5761 ccatggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
     5821 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
     5881 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
     5941 cctggaattc gagctcggta cctttaagac caatgactta caaggcagct gtagatctta
     6001 gccacttttt aaaagaaaag aggggactgg aagggctaat tcactcccaa cgaagacaag
     6061 atatccttga tctgtggatc taccacacac aaggctactt ccctgattag cagaactaca
     6121 caccagggcc aggggtcaga tatccactga cctttggatg gtgctacaag ctagtaccag
     6181 ttgagccaga taaggtagaa gaggccaata aaggagagaa caccagcttg ttacaccctg
     6241 tgagcctgca tgggatggat gacccggaga gagaagtgtt agagtggagg tttgacagcc
     6301 gcctagcatt tcatcacgtg gcccgagagc tgcatccgga gtacttcaag aactgctgat
     6361 atcgagcttg ctacaaggga ctttccgctg gggactttcc agggaggcgt ggcctgggcg
     6421 ggactgggga gtggcgagcc ctcagatcct gcatataagc agctgctttt tgcctgtact
     6481 gggtctctct ggttagacca gatctgagcc tgggagctct ctggctaact agggaaccca
     6541 ctgcttaagc ctcaataaag cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg
     6601 tgtgactctg gtaactagag atccctcaga cccttttagt cagtgtggaa aatctctagc
     6661 agtagtagtt catgtcatct tattattcag tatttataac ttgcaaagaa atgaatatca
     6721 gagagtgaga ggccttgaca ttgctagcgt ttaccgtcga cctctagcta gagcttggcg
     6781 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     6841 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     6901 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     6961 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     7021 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     7081 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     7141 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     7201 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     7261 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     7321 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     7381 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     7441 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     7501 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     7561 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     7621 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     7681 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     7741 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     7801 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     7861 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     7921 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     7981 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     8041 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     8101 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     8161 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     8221 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     8281 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     8341 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     8401 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     8461 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     8521 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     8581 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     8641 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     8701 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     8761 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     8821 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     8881 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     8941 gacgtcgacg gatcgggaga tcaacttgtt tattgcagct tataatggtt acaaataaag
     9001 caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt
     9061 gtccaaactc atcaatgtat cttatcatgt ctggatcaac tggataactc aagctaacca
     9121 aaatcatccc aaacttccca ccccataccc tattaccact gccaattacc tgtggtttca
     9181 tttactctaa acctgtgatt cctctgaatt attttcattt taaagaaatt gtatttgtta
     9241 aatatgtact acaaacttag tagtttttaa agaaattgta tttgttaaat atgtactaca
     9301 aacttagtag t
//
